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sheep antibody against gfap  (R&D Systems)


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    Structured Review

    R&D Systems sheep antibody against gfap
    Sheep Antibody Against Gfap, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sheep+antibody+against+gfap/Human%2FRat+GFAP+Antibody/pmc05864187-255-52-60
    Average 93 stars, based on 26 article reviews
    sheep antibody against gfap - by Bioz Stars, 2026-10
    93/100 stars

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    Staining:

    Article Title: APP upregulation contributes to retinal ganglion cell degeneration via JNK3
    Article Snippet: After three washes with PBS, the sections were mounted in FluorSave reagent (Merk Millipore) and dried overnight in a dark place. .. For the whole-mounted retina staining, after perfusion and post-fixation, the retinae were washed three times in PBS with 0.3% Triton X-100 for 10 min each, and then blocked in 10% normal goat serum and 0.3% Triton for 1 h. Primary mouse antibody against Tuj1 (1:400, catalog no. MMS435P, Covance) [ ] or sheep antibody against GFAP (1:200, catalog no. AF2594, R&D Systems) was added into the blocking solution and incubated over two nights at 4 °C. .. After three 10-min washes in PBS, the retinae were incubated with Alexa Fluor 488 donkey anti-mouse IgG antibody (1:200; Invitrogen) or Alexa Fluor 647 donkey anti-sheep IgG antibody (1:200; Invitrogen) overnight at 4 °C.

    Blocking Assay:

    Article Title: APP upregulation contributes to retinal ganglion cell degeneration via JNK3
    Article Snippet: After three washes with PBS, the sections were mounted in FluorSave reagent (Merk Millipore) and dried overnight in a dark place. .. For the whole-mounted retina staining, after perfusion and post-fixation, the retinae were washed three times in PBS with 0.3% Triton X-100 for 10 min each, and then blocked in 10% normal goat serum and 0.3% Triton for 1 h. Primary mouse antibody against Tuj1 (1:400, catalog no. MMS435P, Covance) [ ] or sheep antibody against GFAP (1:200, catalog no. AF2594, R&D Systems) was added into the blocking solution and incubated over two nights at 4 °C. .. After three 10-min washes in PBS, the retinae were incubated with Alexa Fluor 488 donkey anti-mouse IgG antibody (1:200; Invitrogen) or Alexa Fluor 647 donkey anti-sheep IgG antibody (1:200; Invitrogen) overnight at 4 °C.

    Incubation:

    Article Title: APP upregulation contributes to retinal ganglion cell degeneration via JNK3
    Article Snippet: After three washes with PBS, the sections were mounted in FluorSave reagent (Merk Millipore) and dried overnight in a dark place. .. For the whole-mounted retina staining, after perfusion and post-fixation, the retinae were washed three times in PBS with 0.3% Triton X-100 for 10 min each, and then blocked in 10% normal goat serum and 0.3% Triton for 1 h. Primary mouse antibody against Tuj1 (1:400, catalog no. MMS435P, Covance) [ ] or sheep antibody against GFAP (1:200, catalog no. AF2594, R&D Systems) was added into the blocking solution and incubated over two nights at 4 °C. .. After three 10-min washes in PBS, the retinae were incubated with Alexa Fluor 488 donkey anti-mouse IgG antibody (1:200; Invitrogen) or Alexa Fluor 647 donkey anti-sheep IgG antibody (1:200; Invitrogen) overnight at 4 °C.



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    93
    R&D Systems sheep antibody against gfap
    Sheep Antibody Against Gfap, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sheep+antibody+against+gfap/Human%2FRat+GFAP+Antibody/pmc05864187-255-52-60
    Average 93 stars, based on 1 article reviews
    sheep antibody against gfap - by Bioz Stars, 2026-10
    93/100 stars
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